验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16818-1-AP targets EED in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | EED fusion protein Ag10358 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | embryonic ectoderm development |
| 别名 | WAIT-1, WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein |
| 计算分子量 | 466 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC068995 |
| 基因名称 | EED |
| Gene ID (NCBI) | 8726 |
| RRID | AB_2262065 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75530 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EED antibody 16818-1-AP | Download protocol |
| IHC protocol for EED antibody 16818-1-AP | Download protocol |
| IF protocol for EED antibody 16818-1-AP | Download protocol |
| IP protocol for EED antibody 16818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia | ||
EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. | ||
Eur J Med Chem Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. | ||
J Cell Sci EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT. | ||
Sci Rep Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1. |
