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HADHB Monoclonal antibody, PBS Only

HADHB Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1D12F4

Cat No : 67967-1-PBS

Print datasheet

Synonyms

Acetyl CoA acyltransferase, Beta ketothiolase, ECHB, HADHB, MSTP029, TP BETA

验证数据展示

  • WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HADHB antibody included
  • IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HADHB Ready-To-Use IHC Kit. HADHB primary antibody included.

产品信息

67967-1-PBS targets HADHB in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen HADHB fusion protein Ag30315 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
Synonyms Acetyl CoA acyltransferase, Beta ketothiolase, ECHB, HADHB, MSTP029, TP BETA
Calculated Molecular Weight 51 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC017564
Gene Symbol HADHB
Gene ID (NCBI) 3032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55084
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.