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验证数据展示

  • WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HADHB antibody included
  • IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67967-1-Ig (same clone as 67967-1-PBS)

    WB analysis of HepG2 using 67967-1-Ig (same clone as 67967-1-PBS)

    WB result of HADHB antibody (67967-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HADHB transfected HepG2 cells. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HADHB antibody included
  • IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50546-1

    Cytometric bead array standard curve of MP50546-1

    Cytometric bead array standard curve of MP50546-1, HADHB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67967-1-PBS. Detection antibody: 67967-2-PBS. Standard:Ag30315. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO验证

HADHB Monoclonal antibody, PBS Only (Capture)

HADHB Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67967-1-PBS

产品说明书

CloneNo. 1D12F4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1D12F4, 3-ketoacyl-CoA thiolase, Acetyl CoA acyltransferase, Acetyl-CoA acyltransferase, Beta ketothiolase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HADHB Ready-To-Use IHC Kit. HADHB primary antibody included.

产品信息

67967-1-PBS targets HADHB as part of a matched antibody pair:

MP50546-1: 67967-1-PBS capture and 67967-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag30315

Product name: Recombinant human HADHB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 34-262 aa of BC017564

Sequence: RAAPAVQTKTKKTLAKPNIRNVVVVDGVRTPFLLSGTSYKDLMPHDLARAALTGLLHRTSVPKEVVDYIIFGTVIQEVKTSNVAREAALGAGFSDKTPAHTVTMACISANQAMTTGVGLIASGQCDVIVAGGVELMSDVPIRHSRKMRKLMLDLNKAKSMGQRLSLISKFRFNFLAPELPAVSEFSTSETMGHSADRLAAAFAVSRLEQDEYALRSHSLAKKAQDEGLL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
别名 1D12F4, 3-ketoacyl-CoA thiolase, Acetyl CoA acyltransferase, Acetyl-CoA acyltransferase, Beta ketothiolase
计算分子量 51 kDa
观测分子量47 kDa
GenBank蛋白编号BC017564
基因名称 HADHB
Gene ID (NCBI) 3032
RRIDAB_2918718
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55084
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.

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