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CoraLite® Plus 488-conjugated HIC2 Recombinant antibody

HIC2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230177E8

Cat no : CL488-83162-7

Print datasheet

Synonyms

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

验证数据展示

  • IF Staining of HepG2 using CL488-83162-7

    IF Staining of HepG2 using CL488-83162-7

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HIC2 antibody (CL488-83162-7, Clone: 230177E8 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-83162-7

    FC experiment of HepG2 using CL488-83162-7

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Hic2 Recombinant Antibody (CL488-83162-7, Clone:230177E8)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83162-7-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83162-7 is HIC2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen HIC2 fusion protein Ag18789 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hypermethylated in cancer 2
Synonyms Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2
Calculated Molecular Weight 615 aa, 66 kDa
GenBank Accession NumberBC094787
Gene Symbol HIC2
Gene ID (NCBI) 23119
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96JB3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HIC2 antibody CL488-83162-7Download protocol
Standard Protocols
Click here to view our Standard Protocols