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IHCeasy CHD3 Ready-To-Use IHC Kit

CHD3 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1694

Synonyms

ATP dependent helicase CHD3, CHD 3, CHD3, hZFH, Mi 2a, Mi2 ALPHA, ZFH, Zinc finger helicase

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC1694 (CHD3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC1694 (CHD3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1694 (CHD3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1694 (CHD3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using KHC1694 (CHD3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using KHC1694 (CHD3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using KHC1694 (CHD3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using KHC1694 (CHD3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using KHC1694 (CHD3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using KHC1694 (CHD3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using KHC1694 (CHD3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using KHC1694 (CHD3 IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC1694 is a ready-to-use IHC kit for staining of CHD3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy CHD3 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms ATP dependent helicase CHD3, CHD 3, CHD3, hZFH, Mi 2a, Mi2 ALPHA, ZFH, Zinc finger helicase