IHCeasy GLUT3 Ready-To-Use IHC Kit
GLUT3 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC0090
Synonyms
GLUT 3, GLUT3, SLC2A3
Validation Data Gallery
Product Information
KHC0090 is a ready-to-use IHC kit for staining of GLUT3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy GLUT3 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
GLUT3 (SLC2A3) belongs to the glucose transporter family (GLUTs) which mediates the transport of glucose across cellular membranes in mammalian cells. There are three subclasses within GLUTs: class I comprises the classical transporters GLUT1-4 and GLUT14; class II contains the "odd" isoforms GLUT5, 7, 9, and 11; the isoforms GLUT6, 8, 10, 12 and the proton driven myoinositol transporter HMIT (GLUT13) belong to class III. GLUT3 is considered as a neuron-specific glucose transporter because of its dominant expression in the brain in various species. However, besides the brain GLUT3 is also expressed in tissues with high demand for glucose such as sperm, preimplantation embryos, circulating white blood cells, and an array of carcinoma cell lines.Recently GLUT3 has been identified as a sensitive and specific marker for embyonal carcinomas and yolk tumors.GLUT14 is believed to be the duplicate gene of GLUT3 given to the high identity in sequence between them. Primary antibody in this kit was generated against the internal region of human GLUT3 and may cross-react with GLUT14.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | GLUT 3, GLUT3, SLC2A3 |
