IHCeasy NUPR1 Ready-To-Use IHC Kit
NUPR1 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC1466
Synonyms
Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8
Validation Data Gallery
Product Information
KHC1466 is a ready-to-use IHC kit for staining of NUPR1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy NUPR1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation, and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8 |
