IHCeasy PPP4C Ready-To-Use IHC Kit
PPP4C Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC1002
Synonyms
PP X, PP4, PP4C, PPH3, PPP4, PPP4C, PPX, Protein phosphatase X
Validation Data Gallery
Product Information
KHC1002 is a ready-to-use IHC kit for staining of PPP4C. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy PPP4C Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | PP X, PP4, PP4C, PPH3, PPP4, PPP4C, PPX, Protein phosphatase X |
