验证数据展示
经过测试的应用
Positive FC (Intra) detected in | PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-98005 targets IL-17A in FC (Intra) applications and shows reactivity with mouse samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | mouse |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | interleukin 17A |
别名 | Il17a, Interleukin17A, interleukin 17A, Il17, IL 17A |
计算分子量 | 17 kDa |
GenBank蛋白编号 | NM-010552 |
基因名称 | Il17a |
Gene ID (NCBI) | 16171 |
RRID | AB_3673533 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q62386 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
实验方案
Product Specific Protocols | |
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FC protocol for CL594 IL-17A antibody CL594-98005 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |