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  • Featured Product
  • KD/KO Validated

MCL1 Monoclonal antibody, PBS Only

MCL1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E3C2

Cat No : 66026-1-PBS

Print datasheet

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

验证数据展示

  • WB analysis using 66026-1-Ig (same clone as 66026-1-PBS)

    WB analysis using 66026-1-Ig (same clone as 66026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCL1 antibody (66026-1-Ig, Clone: 1E3C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

    WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

    WB result of MCL1 antibody (66026-1-Ig, 1:1000) with si-Control and si-MCL1 transfected Jurkat cells. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

产品信息

66026-1-PBS targets MCL1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen MCL1 fusion protein Ag10609 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Synonyms EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM
Calculated Molecular Weight 350 aa, 37 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC107735
Gene Symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07820
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.