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  • Featured Product
  • KD/KO Validated

MCL1 Monoclonal antibody, PBS Only (Capture)

MCL1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3C2

Cat no : 66026-1-PBS

Print datasheet

Synonyms

Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3

验证数据展示

  • WB analysis using 66026-1-Ig (same clone as 66026-1-PBS)

    WB analysis using 66026-1-Ig (same clone as 66026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCL1 antibody (66026-1-Ig, Clone: 1E3C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

    WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

    WB result of MCL1 antibody (66026-1-Ig, 1:1000) with si-Control and si-MCL1 transfected Jurkat cells. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • Cytometric bead array standard curve of MP50655-1

    Cytometric bead array standard curve of MP50655-1

    Cytometric bead array standard curve of MP50655-1, MCL1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66026-1-PBS. Detection antibody: 66026-2-PBS. Standard:Ag10609. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

产品信息

66026-1-PBS targets MCL1 as part of a matched antibody pair:

MP50655-1: 66026-1-PBS capture and 66026-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MCL1 fusion protein Ag10609 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Synonyms Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3
Calculated Molecular Weight 350 aa, 37 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC107735
Gene Symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07820
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.