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验证数据展示

  • WB analysis using 84208-2-RR

    WB analysis using 84208-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84208-2-RR (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84208-2-RR

    IHC staining of human colon cancer using 84208-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84208-2-RR (MLH1 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84208-2-RR

    IF Staining of HeLa using 84208-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of HeLa using 84208-2-RR

    IP experiment of HeLa using 84208-2-RR

    IP result of anti-MLH1 (IP:84208-2-RR, 4ug; Detection:84208-2-RR 1:1000) with HeLa cells lysate 1200 ug.

  • IF Staining of HeLa using 84208-2-RR

    IF Staining of HeLa using 84208-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 84208-2-RR

    Affinity and Kinetic Characterization of 84208-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84208-2-RR against Human MLH1 were performed. The affinity constant is below 1 pM.

MLH1 Recombinant antibody

MLH1 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 84208-2-RR

产品说明书

CloneNo. 241472D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HNPCC2, HNPCC, hMLH1, FCC2, COCA2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, A431 cells, SW480 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84208-2-RR targets MLH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 MLH1 fusion protein Ag2319 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
别名 HNPCC2, HNPCC, hMLH1, FCC2, COCA2
计算分子量 756 aa, 85 kDa
观测分子量 85-100 kDa
GenBank蛋白编号BC006850
基因名称 MLH1
Gene ID (NCBI) 4292
RRIDAB_3671764
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band.

实验方案

Product Specific Protocols
WB protocol for MLH1 antibody 84208-2-RRDownload protocol
IHC protocol for MLH1 antibody 84208-2-RRDownload protocol
IF protocol for MLH1 antibody 84208-2-RRDownload protocol
IP protocol for MLH1 antibody 84208-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols