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验证数据展示

  • WB analysis using 15027-1-AP

    WB analysis using 15027-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15027-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15027-1-AP

    IHC staining of mouse testis using 15027-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUP85 antibody included
  • IHC staining of rat brain using 15027-1-AP

    IHC staining of rat brain using 15027-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUP85 antibody included
  • IF Staining of U2OS using 15027-1-AP

    IF Staining of U2OS using 15027-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

NUP85 Polyclonal antibody

NUP85 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15027-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NUP75, Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells
Positive IHC detected inrat brain tissue, human stomach cancer tissue, mouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NUP85 Ready-To-Use IHC Kit. NUP85 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15027-1-AP targets NUP85 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUP85 fusion protein Ag7158 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleoporin 85kDa
别名 NUP75, Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT
计算分子量 75 kDa
观测分子量60 kDa
GenBank蛋白编号BC000697
基因名称 NUP85
Gene ID (NCBI) 79902
RRIDAB_3085449
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW27
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)

实验方案

Product Specific Protocols
WB protocol for NUP85 antibody 15027-1-APDownload protocol
IHC protocol for NUP85 antibody 15027-1-APDownload protocol
IF protocol for NUP85 antibody 15027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols