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ChromoTek Nano-CaptureLigand® human Ig, lambda-LC-specific VHH, biotinylated

The Nano-CaptureLigand® human Ig, lambda-LC captures and immobilizes human Ig lambda light chain (LC) antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human Ig lambda LC antibodies with high affinity and in a site-directed manner.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Human, Ig lambda chain

Cat No : shuLB-1

Synonyms

immunoglobulin, human Ig, lambda-LC, Lambda chain, Lambda light chain

Validation Data Gallery

  • BLI binding kinetics of a human anti-GFP Fab fragment to eGFP. A synthetic human anti-GFP lambda Fab fragment was immobilized using Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of eGFP (ChromoTek EGFP, recombinant protein).

    BLI binding kinetics of a human anti-GFP Fab fragment to eGFP. A synthetic human anti-GFP lambda Fab fragment was immobilized using Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of eGFP (ChromoTek EGFP, recombinant protein).

  • Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of lambda light chain of human Ig antibody (orange). The setup is ready for characterization of human IgG antibodies’ binding kinetics and affinity to substrate (red), human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

    Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of lambda light chain of human Ig antibody (orange). The setup is ready for characterization of human IgG antibodies’ binding kinetics and affinity to substrate (red), human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

  • ELISA capture of a human IgG1 lambda antibody using Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgG1 lambda antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

    ELISA capture of a human IgG1 lambda antibody using Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgG1 lambda antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

  • Species reactivity of Secondary VHH.

    Species reactivity of Secondary VHH.

Product Information

The Nano-CaptureLigand® human Ig, lambda-LC captures and immobilizes human Ig lambda light chain (LC) antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human Ig lambda LC antibodies with high affinity and in a site-directed manner.

DescriptionThe next level of antibody immobilization to BLI and SPR biosensors

• Site-directed and specific immobilization of the Fab-fragment of human Ig through the lambda light chain to streptavidin coated biosensors without biotinylation of the antibody

• Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

• High affinity for stable baseline without significant dissociation

• >10x regeneration for multiple reuse

• High specificity enables human Ig lambda antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

ApplicationsBLI, SPR, ELISA
HostAlpaca
ReactivityHuman Ig, lambda light chain
Does not bind to human Ig kappa; goat IgG; mouse IgG; rabbit IgG Fc; rat IgG
Cross-reactive to rabbit IgG Fab
ConjugateBiotin
Physical StateLiquid
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
Affinity (KD) KD =0.1 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
RRIDAB_2848190
NoteThis product is for research use only, not for diagnostic or therapeutic use