CoraLite® Plus 647-conjugated NeuN Monoclonal antibody

NeuN Monoclonal Antibody for FC (Intra)
Cat No. CL647-66836

产品说明书

CloneNo. 3A4C1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

FC (Intra)

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

This antibody is not suitable for staining in frozen section.

Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66836-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66836 targets NeuN in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 NeuN fusion protein Ag28016 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hexaribonucleotide binding protein 3
别名 cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3
GenBank蛋白编号NM_001082575
基因名称 NeuN
Gene ID (NCBI) 146713
RRIDAB_2935070
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 NeuN antibody CL647-66836Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...