CoraLite® Plus 647-conjugated NeuN Monoclonal antibody
NeuN Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3A4C1
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | SH-SY5Y cells |
Positive FC detected in | SH-SY5Y cells |
This antibody is not suitable for staining in frozen section.
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66836-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66836 targets NeuN in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | NeuN fusion protein Ag28016 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | hexaribonucleotide binding protein 3 |
Synonyms | cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3 |
GenBank Accession Number | NM_001082575 |
Gene Symbol | NeuN |
Gene ID (NCBI) | 146713 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 NeuN antibody CL647-66836 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |