验证数据展示
经过测试的应用
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
Biotin-66836 targets NeuN in IHC applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | IHC Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | NeuN fusion protein Ag28016 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | hexaribonucleotide binding protein 3 |
别名 | cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3 |
GenBank蛋白编号 | NM_001082575 |
基因名称 | NeuN |
Gene ID (NCBI) | 146713 |
RRID | AB_2883076 |
偶联类型 | Biotin |
形式 | Liquid |
纯化方式 | Protein G purification |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
实验方案
Product Specific Protocols | |
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IHC protocol for Biotin NeuN antibody Biotin-66836 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |