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CoraLite® Plus 488-conjugated NeuN Monoclonal antibody

NeuN Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra) and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A4C1

Cat no : CL488-66836

Print datasheet

Synonyms

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

验证数据展示

  • IF Staining of mouse cerebellum using CL488-66836

    IF Staining of mouse cerebellum using CL488-66836

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 NeuN antibody (CL488-66836, Clone: 3A4C1 ) at dilution of 1:200.

  • FC experiment of U-87 MG using CL488-66836

    FC experiment of U-87 MG using CL488-66836

    1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of SH-SY5Y using CL488-66836

    FC experiment of SH-SY5Y using CL488-66836

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inmouse cerebellum tissue, mouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells, U-87 MG cells
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66836-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-66836 targets NeuN in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen NeuN fusion protein Ag28016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexaribonucleotide binding protein 3
Synonyms Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C
GenBank Accession NumberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2883386
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NeuN antibody CL488-66836Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Exp Eye Res

Geranylgeranylacetone-induced heat shock protein70 expression reduces retinal ischemia-reperfusion injury through PI3K/AKT/mTOR signaling

Authors - Lirong Zhang
View Article
mouse

bioRxiv

2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neurons

Authors - Jingcheng Shi
View Article