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CoraLite®555-conjugated NeuN Monoclonal antibody

NeuN Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3A4C1

Cat no : CL555-66836

Print datasheet

Synonyms

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

验证数据展示

  • IF Staining of rat brain using CL555-66836

    IF Staining of rat brain using CL555-66836

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®555 NeuN antibody (CL555-66836, Clone: 3A4C1 ) at dilution of 1:200.

  • FC experiment of U-87 MG using CL555-66836

    FC experiment of U-87 MG using CL555-66836

    1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human NeuN (CL555-66836, Clone:3A4C1) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inU-87 MG cells
Positive FC detected inU-87 MG cells
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66836-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66836 targets NeuN in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen NeuN fusion protein Ag28016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexaribonucleotide binding protein 3
Synonyms cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3
GenBank Accession NumberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2919697
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL555 NeuN antibody CL555-66836Download protocol
Standard Protocols
Click here to view our Standard Protocols