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CoraLite® Plus 488-conjugated P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26714

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells, A431 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDKN1B Ready-To-Use IHC Kit. CDKN1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26714-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26714 targets P27; KIP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 P27; KIP1 fusion protein Ag25083 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1B (p27, Kip1)
别名 CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27
计算分子量 198 aa, 22 kDa
观测分子量 27 kDa
GenBank蛋白编号BC001971
基因名称 p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
RRIDAB_3672803
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46527
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 P27; KIP1 antibody CL488-26714Download protocol
Standard Protocols
Click here to view our Standard Protocols
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