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ChromoTek PARP1-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

Specificity

PARP1

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagRFP

Cat No : xcr

Synonyms

Chromobody, PARP1 Chromobody

Validation Data Gallery

  • Time series of imaging DNA damage in HeLa cells after microirradiation: The PARP1-Chromobody allows real-time monitoring of PARP1 recruitment to the location of DNA damage after microirradiation (yellow triangle) in living cells.

    Time series of imaging DNA damage in HeLa cells after microirradiation: The PARP1-Chromobody allows real-time monitoring of PARP1 recruitment to the location of DNA damage after microirradiation (yellow triangle) in living cells.

Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

DescriptionThe PARP1-Chromobody visualizes endogenous PARP1 in real time in live cells.

• Easily transfect your cells

• Use PARP1-Chromobody® as marker of DNA damage after microirradiation

• PARP1-Chromobody® does not affect cell viability

ApplicationsLive cell imaging
SpecificityPoly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.
Does not bind to PARP2, PARP3, andPARP 9
ReporterTagRFP
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
SequenceWith the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Commun

SPINDOC binds PARP1 to facilitate PARylation.

Authors - Fen Yang
View Article

Proc Natl Acad Sci U S A

Rapid recruitment of p53 to DNA damage sites directs DNA repair choice and integrity.

Authors - Yu-Hsiu Wang
View Article
IF

J Cell Sci

Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus.

Authors - Christian Siebenwirth
View Article
IF

J Biophotonics

Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.

Authors - Simone Pelicci
View Article

Nucleic Acids Res

The loss of DNA polymerase epsilon accessory subunits POLE3-POLE4 leads to BRCA1-independent PARP inhibitor sensitivity

Authors - Hasan Mamar
View Article
Cell treatment

Sci Rep

Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope

Authors - Elisa Longo
View Article