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PCNA Recombinant antibody, PBS Only (Capture)

PCNA Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240306C1

Cat no : 81302-3-PBS

Print datasheet

Synonyms

proliferating cell nuclear antigen, Cyclin, 240306C1

验证数据展示

  • Sandwich ELISA standard curve of MP00424-4

    Sandwich ELISA standard curve of MP00424-4

    Sandwich ELISA standard curve of MP00424-4, human PCNA Recombinant Matched Antibody Pair - PBS only. 81302-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Ag0277. 81302-4-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Sample test of MP00424-4

    Sample test of MP00424-4

    The mean PCNA concentration was determined to be 1,050.8 ng/mL in Jurkat cell extract based on a 5.8 mg/mL extract load, 312.4 ng/mL in MCF-7 cell extract based on a 3.1 mg/mL extract load and 276.5 ng/mL in Hela cell extract based on a 3.6 mg/mL extract load.

  • Cytometric bead array standard curve of MP00424-2

    Cytometric bead array standard curve of MP00424-2

    Cytometric bead array standard curve of MP00424-2, PCNA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81302-3-PBS. Detection antibody: 81302-4-PBS. Standard: Ag0277. Range: 1.56-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy PCNA Ready-To-Use IHC Kit. PCNA primary antibody included.

产品信息

81302-3-PBS targets PCNA as part of a matched antibody pair:

MP00424-2: 81302-3-PBS capture and 81302-4-PBS detection (validated in Cytometric bead array)

MP00424-4: 81302-3-PBS capture and 81302-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen PCNA fusion protein Ag0277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proliferating cell nuclear antigen
Synonyms proliferating cell nuclear antigen, Cyclin, 240306C1
Calculated Molecular Weight 29 kDa/31 kDa
GenBank Accession NumberBC000491
Gene Symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C