|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10753-1-AP targets TIMP1 in IHC, IF, ELISA applications and shows reactivity with human samples.
|Tested Applications||IF, IHC,ELISA|
|Cited Applications||IF, IHC|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TIMP1 fusion protein Ag1146|
|Full Name||TIMP metallopeptidase inhibitor 1|
|Synonyms||CLGI, Collagenase inhibitor, EPA, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1, TIMP-1|
|Calculated molecular weight||23 kDa|
|GenBank accession number||BC007097|
|Uniprot||P01033||Gene ID (NCBI)||7076|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tissue Inhibitor of Metalloproteinases-1 (TIMP1) is a naturally occurring protein in the human organism regulating the activity of matrix metalloproteinases (MMPs). TIMP1 encodes a 931 base-pair mRNA and a 207 amino acid protein. Overexpression of TIMP1 can lead to a substantially increase of genes involved in proliferation, apoptosis and signal transduction. In addition, TIMP1 was found to decrease tumor cell sensitivity to multiple anticancer drugs by activation of downstream pathways and exhibited anti-apoptotic activity. Specifically, the TIMP1 could degrade cyclinB1 and activate the NF-kβ signaling pathway to protect breast cancer cells against chemotherapy-induced cell death.
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