验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, HEK-293T cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, mouse thymus tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21402-1-AP targets UHRF1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16111 Product name: Recombinant human UHRF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-392 aa of BC113875 Sequence: QSLVLPHSTKERDSELSDTDSGCCLGQSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGAWFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGPSCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLSSVPSEDEWYCPECRNDASEVVLAGERLRE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-like with PHD and ring finger domains 1 |
| 别名 | E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1, HuNp95 |
| 计算分子量 | 806 aa, 91 kDa |
| 观测分子量 | 91-100 kDa |
| GenBank蛋白编号 | BC113875 |
| 基因名称 | UHRF1 |
| Gene ID (NCBI) | 29128 |
| RRID | AB_10860902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96T88 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for UHRF1 antibody 21402-1-AP | Download protocol |
| IHC protocol for UHRF1 antibody 21402-1-AP | Download protocol |
| IP protocol for UHRF1 antibody 21402-1-AP | Download protocol |
| WB protocol for UHRF1 antibody 21402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Methionine restriction promotes cGAS activation and chromatin untethering through demethylation to enhance antitumor immunity
| ||
Nat Cell Biol Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination | ||
Cell Discov USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.
| ||
Nucleic Acids Res SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1. | ||
Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. | ||
Adv Sci (Weinh) HDAC2-Mediated METTL3 Delactylation Promotes DNA Damage Repair and Chemotherapy Resistance in Triple-Negative Breast Cancer |
