• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66452-1-Ig (same clone as 66452-1-PBS)

    WB analysis using 66452-1-Ig (same clone as 66452-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66452-1-Ig (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    IHC staining of human pancreas cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    IHC staining of human pancreas cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66452-1-Ig (same clone as 66452-1-PBS)

    IF Staining of MCF-7 using 66452-1-Ig (same clone as 66452-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    IF Staining of human breast cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    IF Staining of human breast cancer using 66452-1-Ig (same clone as 66452-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66452-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ZO-1 Monoclonal antibody, PBS Only

ZO-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66452-1-PBS

产品说明书

CloneNo. 1G4A1

宿主/亚型

Mouse / IgG1

种属反应性

human, canine

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66452-1-PBS targets ZO-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human, canine
免疫原 ZO-1 fusion protein Ag16454 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tight junction protein 1 (zona occludens 1)
别名 TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1
计算分子量 1748 aa, 195 kDa
观测分子量 230 kDa
GenBank蛋白编号BC111712
基因名称 ZO-1
Gene ID (NCBI) 7082
RRIDAB_2881821
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07157
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).