CoraLite®555-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody

Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF/ICC
Cat No. CL555-66200

产品说明书

CloneNo. 7E5H8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, canine

应用

IF/ICC

Ac Tubulin (Lys40), Ac Tubulin Lys40, Acetyl Tubulin, Acetyl Tubulin (Lys40), Acetyl Tubulin Lys40

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy TUBA1A Ready-To-Use IHC Kit. TUBA1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66200-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66200 targets Acetyl-Tubulin (Lys40) in IF/ICC applications and shows reactivity with human, mouse, rat, pig, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig, canine
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, alpha 1a
别名 Ac Tubulin (Lys40), Ac Tubulin Lys40, Acetyl Tubulin, Acetyl Tubulin (Lys40), Acetyl Tubulin Lys40
计算分子量 52 kDa
观测分子量 50-55 kDa
GenBank蛋白编号NM_006009
基因名称 Alpha Tubulin
Gene ID (NCBI) 7846
RRIDAB_2919679
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ71U36
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

 

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

 

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

 

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

 

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

 

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

 

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).


实验方案

Product Specific Protocols
IF protocol for CL555 Acetyl-Tubulin (Lys40) antibody CL555-66200Download protocol
Standard Protocols
Click here to view our Standard Protocols
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