Acetyl-Tubulin (Lys40) Monoclonal antibody

Acetyl-Tubulin (Lys40) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66200-1-Ig

产品说明书

CloneNo. 7E5H8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, canine and More (4)

应用

WB, IHC, IF/ICC, ELISA and More (1)

acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, 7E5H8, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis
Positive IHC detected inmouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells
Positive ELISA detected inRecombinant protein
Planning an IHC experiment? We recommend our IHCeasy TUBA1A Ready-To-Use IHC Kit. TUBA1A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66200-1-Ig targets Acetyl-Tubulin (Lys40) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig, canine
文献引用反应性human, mouse, rat, pig, rabbit, canine, zebrafish, bovine, drosophila
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, alpha 1a
别名 acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, 7E5H8, EC:3.6.5.-
计算分子量 52 kDa
观测分子量 50-55 kDa
GenBank蛋白编号NM_006009
基因名称 Alpha Tubulin
Gene ID (NCBI) 7846
RRIDAB_2722562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ71U36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

 

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

 

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

 

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

 

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

 

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

 

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).


实验方案

Product Specific Protocols
WB protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-IgDownload protocol
IHC protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-IgDownload protocol
IF protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Stem Cell

Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.

Authors - Aleksandra Tata
mouseIF

Adv Sci (Weinh)

DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion

Authors - Xulong Ding

Adv Sci (Weinh)

Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles

Authors - Pei Jiang
humanIF

J Clin Invest

Nasal ciliated cells are primary targets for SARS-CoV-2 replication in early stage of COVID-19.

Authors - Ji Hoon Ahn
mouseWB

Aging Cell

SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.

Authors - Yingying Zhao
WB

Biomaterials

A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions.

Authors - Ji Wang
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