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验证数据展示

  • WB analysis using 66200-1-Ig (same clone as 66200-1-PBS)

    WB analysis using 66200-1-Ig (same clone as 66200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66200-1-Ig (Acetyl-Tubulin (Lys40) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66200-1-PBS in a different storage buffer formulation.

  • IF Staining of islet using 66200-1-Ig (same clone as 66200-1-PBS)

    IF Staining of islet using 66200-1-Ig (same clone as 66200-1-PBS)

    (left) Confocal image of a single-plane cross-section of a healthy human islet, showing primary cilia on both alpha cells (glucagon, red) and non-alpha cells (non-red). Acetylated alpha tubulin, cilia (green, Cat. No 66200-1-Ig, 1:400), nuclei (blue), scale 20 μm. (right) Primary cilia in wildtype B6 mouse islets: acetylated alpha tubulin (green, Cat. No 66200-1-Ig, 1:400), glucagon (red), nuclei (blue), single-plane images, scales 10 μm. (Fig from Dr. Jing Hughes) This data was developed using the same antibody clone with 66200-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 66200-1-Ig (same clone as 66200-1-PBS)

    IHC staining of mouse ovary using 66200-1-Ig (same clone as 66200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66200-1-Ig (Acetyl-Tubulin (Lys40) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66200-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBA1A antibody included
  • ELISA experiment of Recombinant protein using 66200-1-Ig (same clone as 66200-1-PBS)

    ELISA experiment of Recombinant protein using 66200-1-Ig (same clone as 66200-1-PBS)

    Specificity validation by indirect ELISA. Indirect ELISA was carried out by coating E.coli expressed GST-tagged Tubulin alpha (70 ng/well), non-acetylated Tubulin alpha, and Lys40 acetylated Tubulin alpha (with excessive amount) followed by blocking and adding serial diluted GST tag primary antibody (Cat.NO. 66001-2-Ig) and Acetylated Tubuliln antibody 66200-1-Ig respectively. HRP-Goat anti-mouse secondary antibody was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 66200-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 66200-1-Ig (same clone as 66200-1-PBS)

    IF Staining of MDCK using 66200-1-Ig (same clone as 66200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 66200-1-PBS in a different storage buffer formulation.

Acetyl-Tubulin (Lys40) Monoclonal antibody, PBS Only

Acetyl-Tubulin (Lys40) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA, Indirect ELISA
Cat No. 66200-1-PBS

产品说明书

CloneNo. 7E5H8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, canine

应用

WB, IHC, IF/ICC, ELISA, Indirect ELISA

别名

TUBA1A, Tubulin alpha 1A chain, TUBA3, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TUBA1A Ready-To-Use IHC Kit. TUBA1A primary antibody included.

产品信息

66200-1-PBS targets Acetyl-Tubulin (Lys40) in WB, IHC, IF/ICC, ELISA, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

经测试应用 WB, IHC, IF/ICC, ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, canine
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, alpha 1a
别名 TUBA1A, Tubulin alpha 1A chain, TUBA3, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain
计算分子量 52 kDa
观测分子量 50-55 kDa
GenBank蛋白编号NM_006009
基因名称 Alpha Tubulin
Gene ID (NCBI) 7846
RRIDAB_2722562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ71U36
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

 

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

 

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

 

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

 

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

 

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

 

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).