验证数据展示
经过测试的应用
| Positive IF-Fro detected in | mouse kidney tissue |
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 3 publications below |
产品信息
CL488-66200 targets Acetyl-Tubulin (Lys40) in IF/ICC, IF-Fro applications and shows reactivity with human, mouse, rat, pig, canine samples.
| Tested Applications | IF/ICC, IF-Fro Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, pig, canine |
| Cited Reactivity | human, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tubulin, alpha 1a |
| Synonyms | acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, 7E5H8, Detyrosinated tubulin alpha-1A chain |
| Calculated Molecular Weight | 52 kDa |
| GenBank Accession Number | NM_006009 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 7846 |
| RRID | AB_2883282 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q71U36 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Acetyl-Tubulin (Lys40) antibody CL488-66200 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
STAR Protoc Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells | ||
BBA Adv The dynamic nature of the coronavirus receptor, angiotensin-converting enzyme 2 (ACE2) in differentiating airway epithelia. |