CoraLite®594-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody

Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF/ICC
Cat No. CL594-66200

产品说明书

CloneNo. 7E5H8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, Canine, pig and More (1)

应用

IF/ICC and More (1)

Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, acetylated Tubulin (Lys40), acetylated Tubulin(Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, B ALPHA 1, LIS3, TUBA1A, TUBA1A(ace-40Lys), TUBA3, tubulin, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy TUBA1A Ready-To-Use IHC Kit. TUBA1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66200-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 3 publications below

产品信息

CL594-66200 targets Acetyl-Tubulin (Lys40) in IF/ICC applications and shows reactivity with human, mouse, rat, Canine, pig samples.

经测试应用 IF/ICC Application Description
文献引用应用IF
经测试反应性 human, mouse, rat, Canine, pig
文献引用反应性zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, alpha 1a
别名 Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, acetylated Tubulin (Lys40), acetylated Tubulin(Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, B ALPHA 1, LIS3, TUBA1A, TUBA1A(ace-40Lys), TUBA3, tubulin, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a
计算分子量 52 kDa
GenBank蛋白编号NM_006009
基因名称 Alpha Tubulin
Gene ID (NCBI) 7846
RRIDAB_2883533
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ71U36
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549) And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

实验方案

Product Specific Protocols
IF protocol for CL594 Acetyl-Tubulin (Lys40) antibody CL594-66200Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

J Neurosci

Comparison of ciliary targeting of two rhodopsin-like GPCRs: role of C-terminal localization sequences in relation to cilium type.

Authors - Abhishek Chadha
IF

Curr Biol

Cones and cone pathways remain functional in advanced retinal degeneration

Authors - Erika M Ellis
zebrafishIF

Cell Biosci

IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1

Authors - Hye In Ka
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