GST Tag Polyclonal antibody

GST Tag Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

FC, WB, ELISA and More (4)

Conjugate

Unconjugated

Cat No : 10000-0-AP

Print datasheet

Synonyms

Glutathione S-Transferase, GST, gst tag



经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10000-0-AP targets GST Tag in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications FC, WB, ELISA
Cited Applications ChIP, CoIP, ELISA, IF, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GST Tag
Synonyms Glutathione S-Transferase, GST, gst tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_11042316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

发表文章

SpeciesApplicationTitle
humanWB,IP

Signal Transduct Target Ther

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Authors - Saisai Guo
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang

Ann Rheum Dis

Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B

Authors - Sherri Longobardi
WB

Immunity

Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection.

Authors - Jun Xiao
humanWB

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
WB,IP

Nat Struct Mol Biol

CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames

Authors - Caishang Zheng