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ChromoTek iST V5-Trap® Kit

Kit for IP/ Co-IP of V5-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

V5-Tag

Applications

IP, MS

Conjugate

Agarose

Type

Nanobody

Cat No : v5tak-iST

Synonyms

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

Validation Data Gallery

  • Schematic workflow of iST Nano-Trap® Kit

    Schematic workflow of iST Nano-Trap® Kit

  • Save up to 40 hours on your mass spec experiments with the iST Nano-Trap® Kits

    Save up to 40 hours on your mass spec experiments with the iST Nano-Trap® Kits

Product Information

Kit for IP/ Co-IP of V5-tagged proteins & sample preparation for mass spectrometry (MS)

DescriptionThe iST V5-Trap® Kit enables researchers to process V5-tagged proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek V5-Trap for immunoprecipitation/Co-IP of V5-tagged proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

• Reliable identification of V5-tagged proteins & interacting partners by mass spec

• Superior processing of IP samples into clean peptide mixtures

• Intuitive protocol and reproducible results

ApplicationsIP, MS
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion.
Storage Buffer20% ethanol
Storage ConditionKit is shipped at ambient temperature. Upon receipt store V5-Trap at 4°C and Digest at -20°C

Kit components

ComponentDescription
V5-Trap™ Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.