ChromoTek iST mNeonGreen-Trap Kit for AP-MS sample preparation of mNeonGreen-fusion proteins

Kit for IP/ Co-IP of mNeonGreen tagged proteins and sample preparation for mass spectrometry (MS)

Specificity

mNeonGreen

Applications

IP, MS

Conjugate

Agarose

Type

Nanobody

Cat No : ntak-iST

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag



Product Information

Kit for IP/ Co-IP of mNeonGreen tagged proteins and sample preparation for mass spectrometry (MS)

DescriptionThe iST mNeonGreen-Trap Kit enables researchers to process mNeonGreen-tagged proteins and their interacting partners for MS analysis by including the mNeonGreen-Trap for immunoprecipitation/Co-IP of mNeonGreen-tagged proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

• Reliable dentification of mNeonGreen-fusion proteins & interacting partners by MS

• Identification of even low abundant/ low expressed mNeonGreen-fusion proteins

• Superior processing of IP samples into clean peptide mixtures

• Intuitive protocol for reproducible results

ApplicationsIP, MS
Specificity/TargetmNeonGreen
Binding capacity30 μg of recombinant mNeonGreen per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe mNeonGreen-Trap is optimized for on-bead digestion.
Storage Buffer20% EtOH
Storage ConditionKit is shipped at ambient temperature. Upon receipt store mNeonGreen-Trap at 4°C and Digest at -20°C

Kit components

ComponentDescription
mNeonGreen-Trap Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.

Publications

ApplicationTitle
CoIP

Fungal Genet Biol

Identification and characterisation of sPEPs in Cryptococcus neoformans.

Authors - Paige E Erpf