验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81615 targets pan-RAS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | pan-RAS fusion protein Ag2700 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| 别名 | KRAS, KRAS4A, KRAS4B, pan RAS, EC:3.6.5.2 |
| 计算分子量 | 188 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC013572 |
| 基因名称 | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_3673051 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01116 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 pan-RAS antibody CL488-81615 | Download protocol |
| FC protocol for CL Plus 488 pan-RAS antibody CL488-81615 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |