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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-4 protein- GMP grade

EC50

6-30 ng/mL in NIH 3T3 cells in defined media

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1218-GMP

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Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

Validation Data Gallery

  • Purity of recombinant human FGF-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human GMP FGF-4 (HZ-1218-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-4 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated assay. The EC50 values range from 6-30 ng/mL.

    Recombinant human GMP FGF-4 (HZ-1218-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-4 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated assay. The EC50 values range from 6-30 ng/mL.

Technical Specifications

GeneID 2249
Species Human
Expression HEK293
EC50 6-30 ng/mL in NIH 3T3 cells in defined media
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP11403
Molecular Mass 17 and 27 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Fibroblast growth factor-4 (FGF-4) plays an important physiologically role in the self-renewal of human embryonic stem cells (PMID 21990129). It also promotes stem cell proliferation and may aid in differentiation. Xeno-free Recombinant Human FGF-4 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 17 and 27 kDa.

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3