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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-8b protein- GMP grade

EC50

10-60 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1103-GMP

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Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, FGF-8b, Fibroblast growth factor 8, HBGF 8, KAL6, FGF8b

Validation Data Gallery

  • Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human FGF-8b (HZ-1103-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.

    GMP Recombinant human FGF-8b (HZ-1103-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.

Technical Specifications

GeneID 2253
Species Human
Expression HEK293
EC50 10-60 ng/mL EC50
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP55075
Molecular Mass 30 to 45 kDa reduced and non-reduced, monomer, glycosylated
Formulation 10 mM Tris-HCl pH 7.4 + 800 mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Fibroblast growth factor 8 (FGF8), also known as AIGF and HBGF-8, belongs to the FGF family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (PMID: 11276432; 15863033). FGF8 stimulates growth in an autocrine manner in normal individuals but mediates hormonal action during tumorigenesis (PMID 17512240). Defects in FGF8 cause Kallmann syndrome type 6 (KAL6) and idiopathic hypogonadotropic hypogonadism (IHH) (PMID 18596921).

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, FGF-8b, Fibroblast growth factor 8, HBGF 8, KAL6, FGF8b

Publications

SpeciesTitle
mouse

Front Cell Neurosci

Nicotinamide enhances myelin production after demyelination through reduction of astrogliosis and microgliosis

Authors - Stefanos Ioannis Kaplanis
View Article