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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN beta protein- GMP grade

EC50

0.015-0.08 ng/mL

Specific Activity

minimally 1.00 X 109 IU/mg, typically 1.56 X 109 IU/mg

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1298-GMP

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Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

Validation Data Gallery

  • Purity of GMP recombinant human IFN beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP recombinant human IFN beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human IFN beta (HZ-1298-GMP) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.015-0.08 ng/mL​.

    GMP Recombinant human IFN beta (HZ-1298-GMP) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.015-0.08 ng/mL​.

Technical Specifications

GeneID 3456
Species Human
Expression HEK293
EC50 0.015-0.08 ng/mL
Specific Activityminimally 1.00 X 109 IU/mg, typically 1.56 X 109 IU/mg
Purity > 95%
Endotoxin<0.1 EU/μg
Accession NumberP01574
Molecular Mass 21 to 24 kDa reduced, 20 to 23 and 38 to 42 non-reduced, glycosylated
Formulation Sodium Acetate pH 4.8 + 150mM NaCl + CHAPS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IFN beta is a member of type I family of interferons. It binds to the IFNα/β receptor (IFNAR), activating the Jak/ STAT pathway. Activation of this signaling pathway results in upregulating genes that inhibit viral infection and regulate MHC class I antigens. It is primarily produced by fibroblasts and monocytes. In addition to inhibiting viral infection, IFN beta is also involved in regulating and activating immune response against bacteria, parasite and tumor cells. Multiple sclerosis is characterized by a deficiency of IFN beta 1. An injectable form of IFN beta 1 is used for MS treatment (PMID: 22222875; 29258190; 8602746; 8469318).

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast