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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TGF beta 1 protein- GMP grade

EC50

0.01-0.17 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg, typically 1.24 x 107 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1011-GMP

Print datasheet

Synonyms

CED, DPD1, LAP, TGF ?1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Validation Data Gallery

  • Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

    Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

  • Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

    Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

Technical Specifications

GeneID 7040
Species Human
Expression HEK293
EC50 0.01-0.17 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg, typically 1.24 x 107 IU/mg
Purity >95%
Endotoxin<0.1 EU/ug
Accession NumberP01137
Molecular Mass 13 kDa reduced, 25 kDa non-reduced, homodimer, non-glycosylated
Formulation 50 mM NaOAc pH 3.7, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, and cell differentiation. TGF acts synergistically with TGFA in inducing transformation process. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB having specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling being a potent stimulator of an osteoblastic bone formation (PMID: 11586292).

Synonyms

CED, DPD1, LAP, TGF ?1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Publications

SpeciesTitle
human

PLoS One

PNPLA3 downregulation exacerbates the fibrotic response in human hepatic stellate cells

Authors - Brian Rady
View Article