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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BMP-4 protein- GMP grade

EC50

1.5-9 ng/mL

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1045-GMP

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Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

Validation Data Gallery

  • Purity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human BMP-4 (HZ-1045-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

    Recombinant human BMP-4 (HZ-1045-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

Technical Specifications

GeneID 652
Species Human
Expression HEK293
EC50 1.5-9 ng/mL
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP12644
Molecular Mass 20 to 24 kDa reduced, 32 to 38 kDa non-reduced, homodimer, glycosylated
Formulation 2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Bone morphogenetic protein-4 (BMP-4), also known as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP-4 can also induce cartilage and bone formation (PMID 16234975). Xeno-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa.

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME