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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Pleiotrophin (PTN) protein

Activity

100-500 ng/mL

Species Reactivity

human,rat

Purity

>95%

Cat No : HZ-1278

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Synonyms

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN

Validation Data Gallery

  • Purity of recombinant human PTN was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human PTN was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human PTN (HZ-1278) stimulates dose-dependent proliferation of the THP-1 monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant PTN for 120 hours. The EC50 range is 100-500 ng/mL​.

    Recombinant human PTN (HZ-1278) stimulates dose-dependent proliferation of the THP-1 monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant PTN for 120 hours. The EC50 range is 100-500 ng/mL​.

Technical Specifications

GeneID 5764
Species Human
Expression HEK293
Activity 100-500 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP21246
Molecular Mass 18-21 kDa reduced, 17 to 20 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Pleiotrophin is a secreted growth factor that induces neurite outgrowth being mitogenic for fibroblasts, epithelial, and endothelial cells. It binds anaplastic lymphoma kinase (ALK) inducing MAPK pathway activation, as an important step in the anti-apoptotic signaling of PTN and cell proliferation (PMID:1768439; 1733956; 11278720)

Synonyms

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN