Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-S6 Ribosomal protein (Ser236) (80206-1-RR, Clone:7K17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80206-1-PBS in a different storage buffer formulation.

1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-S6 Ribosomal protein (Ser236) (80206-1-RR, Clone:7K17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80206-1-PBS in a different storage buffer formulation.

Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29220-1-AP (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.