S6 Ribosomal protein Recombinant antibody
S6 Ribosomal protein Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse, Zebrafish and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
6L9
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, RAW 264.7 cells, zebrafish tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
80208-1-RR targets S6 Ribosomal protein in WB, IF, ELISA applications and shows reactivity with Human, rat, mouse, Zebrafish samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, rat, mouse, Zebrafish |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ribosomal protein S6 |
| Synonyms | 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC000524 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2918876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S6 Ribosomal protein antibody 80208-1-RR | Download protocol |
| IF protocol for S6 Ribosomal protein antibody 80208-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells | ||
Biomark Res Deciphering metabolic heterogeneity in retinoblastoma unravels the role of monocarboxylate transporter 1 in tumor progression |
