CoraLite® Plus 488-conjugated S6 Ribosomal protein Recombinant antibody

S6 Ribosomal protein Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse, zabrafish

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6L9

Cat no : CL488-80208

Print datasheet

Synonyms

40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6



经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 80208-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80208 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, rat, mouse, zabrafish samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, rat, mouse, zabrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms 40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 S6 Ribosomal protein antibody CL488-80208Download protocol
Standard Protocols
Click here to view our Standard Protocols