CoraLite® Plus 488-conjugated S6 Ribosomal protein Recombinant antibody
S6 Ribosomal protein Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse, zabrafish
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6L9
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80208-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80208 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, rat, mouse, zabrafish samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, rat, mouse, zabrafish |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ribosomal protein S6 |
| Synonyms | 40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC000524 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 S6 Ribosomal protein antibody CL488-80208 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |