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Phospho-S6 Ribosomal protein (Ser236) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser236) Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

7K17

Cat no : 80206-1-PBS

Print datasheet

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

验证数据展示

  • WB analysis using 80206-1-RR (same clone as 80206-1-PBS)

    WB analysis using 80206-1-RR (same clone as 80206-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80206-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80206-1-RR (same clone as 80206-1-PBS)

    FC experiment of HEK-293 using 80206-1-RR (same clone as 80206-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-S6 Ribosomal protein (Ser236) (80206-1-RR, Clone:7K17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80206-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

产品信息

80206-1-PBS targets Phospho-S6 Ribosomal protein (Ser236) in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity mouse, human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2918875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 80206-1-RR specifically recognizes the phosphorylation site of Ser236 or dual site Ser235/236.