Phospho-S6 Ribosomal protein (Ser236) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser236) Recombinant Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7K17

Cat No : 80206-1-RR

Print datasheet

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser236), Phospho-S6 Ribosomal protein (Ser236), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein



经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, Calyculin A treated HEK-293 cells, IGF-1 treated MCF-7 cells
Positive FC detected inCalyculin A treated HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80206-1-RR targets Phospho-S6 Ribosomal protein (Ser236) in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser236), Phospho-S6 Ribosomal protein (Ser236), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2918875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 80206-1-RR specifically recognizes the phosphorylation site of Ser236 or dual site Ser235/236.

实验方案

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser236) antibody 80206-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols