Cell culture workflow

Cell culture workflow in 4 simple steps

ISOLATE

• Separate fresh tissue of interest.

• Treat tissue with enzyme(s) (e.g. trypsin, collagenase, protease) and/or mechanically to isolate cells.

• Wash, count, and seed cells.

VERIFY

• Examine cells under a brightfield microscope to assess their growth state, attachment to culture vessels/flasks, and to check for any signs of infection.

• Monitor cells for the following days until they reach confluence.

• Verify isolated cell types by their morphology and expressed biomarkers.

CULTURE

• Passage cells to propagate the cell line. 

• Make master and working cell banks. 

• Immortalize cells if necessary.

INVESTIGATE

• Plan and execute experiments.

• Keep monitoring cell state and possible infections using a brightfield microscope.