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Standard Workflow

Labeling 0.5µg of primary antibody  

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Workflow

 

Before you begin, equilibrate all reagents to room temperature

  

Procedure:  
  1. Combine 0.5µg of primary antibody with 1µL of FlexLinker. Add FlexBuffer to bring total volume to 8µL.
  2. Mix gently and incubate for 5 minutes at room temperature in the dark.
  3. Add 2µL of FlexQuencher.
  4. Mix gently and incubate for 5 minutes at room temperature in the dark.
  5. The antibody is now ready to be used.
  
Scale up proportionately to label larger quantities of primary antibody 

 

Download the Protocol as PDF