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  • KD/KO Validated

COX4I2 Polyclonal antibody

COX4I2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11463-1-AP

Print datasheet

Synonyms

COX IV 2, COX4, COX4 2, COX4B, COX4I2, COX4L2, COXIV 2



经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, MCF-7 cells, human heart tissue, human lung tissue, A375 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11463-1-AP targets COX4I2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen COX4I2 fusion protein Ag1987 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase subunit IV isoform 2 (lung)
Synonyms COX IV 2, COX4, COX4 2, COX4B, COX4I2, COX4L2, COXIV 2
Calculated Molecular Weight 171 aa, 20 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC057779
Gene Symbol COX4I2
Gene ID (NCBI) 84701
RRIDAB_2085287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.

实验方案

Product Specific Protocols
WB protocol for COX4I2 antibody 11463-1-APDownload protocol
IHC protocol for COX4I2 antibody 11463-1-APDownload protocol
IF protocol for COX4I2 antibody 11463-1-APDownload protocol
IP protocol for COX4I2 antibody 11463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.

Authors - Arpita Chowdhury
humanWB

Oncotarget

Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.

Authors - Claudia R Oliva
  • KD Validated
mouseIF

Sci Signal

Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.

Authors - Alejandro Moreno-Domínguez
  • KO Validated
humanWB

Free Radic Biol Med

Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma.

Authors - Md Yousuf Ali
humanWB

Bioconjug Chem

GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery.

Authors - Theodoros Karampelas
humanWB

Free Radic Biol Med

Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.

Authors - Schneider Lonnie L