验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, NIH/3T3 cells, rat liver tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11460-1-AP targets COX6A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag2006 Product name: Recombinant human COX6A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-109 aa of BC007723 Sequence: MAVVGVSSVSRLLGRSRPQLGRPMSSGAHGEEGSARMWKTLTFFVALPGVAVSMLNVYLKSHHGEHERPEFIAYPHLRIRTKPFPWGDGNHTLFHNPHVNPLPTGYEDE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome c oxidase subunit VIa polypeptide 1 |
| 别名 | COX VIa L, COX VIa-L, COX6AL, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver |
| 计算分子量 | 85 aa, 9 kDa |
| 观测分子量 | 9.5 kDa |
| GenBank蛋白编号 | BC007723 |
| 基因名称 | COX6A1 |
| Gene ID (NCBI) | 1337 |
| RRID | AB_2085445 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12074 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX6A1(Cytochrome c oxidase subunit 6A1, mitochondrial) is also named as COX6AL and belongs to the cytochrome c oxidase subunit 6A family.This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX6A1 antibody 11460-1-AP | Download protocol |
| IHC protocol for COX6A1 antibody 11460-1-AP | Download protocol |
| IF protocol for COX6A1 antibody 11460-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1).
| ||
Elife Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome. | ||
Cell Death Dis O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma. | ||
Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV | ||
Hum Mol Genet Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation. | ||
Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV. |
