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EPCAM/CD326 Polyclonal antibody
EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse colon tissue, rat colon tissue |
| Positive IHC detected in | human colon cancer tissue, mouse colon tissue, human breast cancer tissue, human colon tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue, MCF-7 cells, mouse lung tissue |
| Positive IF-Fro detected in | mouse colon tissue |
| Positive IF/ICC detected in | HT-29 cells, MCF-7 cells |
| Positive FC detected in | HT-29 cells |
Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21050-1-AP targets EPCAM/CD326 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, FC, chIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | EPCAM/CD326 fusion protein Ag15393 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | epithelial cell adhesion molecule |
| Synonyms | EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1 |
| Calculated Molecular Weight | 314 aa, 35 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC014785 |
| Gene Symbol | EPCAM |
| Gene ID (NCBI) | 4072 |
| RRID | AB_10693684 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16422 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPCAM/CD326 antibody 21050-1-AP | Download protocol |
| IHC protocol for EPCAM/CD326 antibody 21050-1-AP | Download protocol |
| IF protocol for EPCAM/CD326 antibody 21050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma | ||
Cancer Cell Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression. | ||
Gut Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis. | ||
Nat Commun Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma. | ||
Biosens Bioelectron Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier. | ||
EMBO J A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. |
