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EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21050-1-AP

Print datasheet

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1



经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue, human breast cancer tissue, human colon tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse colon tissue, MCF-7 cells, mouse lung tissue
Positive FC detected inHT-29 cells, HepG2 cells, A431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21050-1-AP targets EPCAM/CD326 in WB, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications chIP, FC, IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen EPCAM/CD326 fusion protein Ag15393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_10693684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16422
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 21050-1-APDownload protocol
IHC protocol for EPCAM/CD326 antibody 21050-1-APDownload protocol
IF protocol for EPCAM/CD326 antibody 21050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cancer

m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma

Authors - Han Fang
mouseIHC

Cancer Cell

Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.

Authors - Chunxiao Xu
mouseIF

Gut

Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.

Authors - Yunben Yang
humanIF

Nat Commun

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Authors - Zhaohui Chen
humanWB

Biosens Bioelectron

Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.

Authors - Hao Lu
humanWB

EMBO J

A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.

Authors - Doowon Huh