• 概述
  • 经过测试的应用
  • 产品信息
  • 发表文章 (677)
  • 实验方案
  • 相关产品 (8)
  • Featured Product
  • KD/KO Validated

c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 10828-1-AP

Print datasheet

Synonyms

MYC, Myc proto oncogene protein, MRTL, Class E basic helix-loop-helix protein 39, c Myc



经过测试的应用

Positive WB detected inHT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells.
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells, HEK-293 cells, U2OS cells
Positive FC (Intra) detected inHeLa cells

This antibody is not for the detetion of MYC-tag.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10828-1-AP targets c-MYC in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, EMSA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF, IP, CoIP, chIP, EMSA
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, chicken, sheep, p. lilacinum
Immunogen c-MYC fusion protein Ag1263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Synonyms MYC, Myc proto oncogene protein, MRTL, Class E basic helix-loop-helix protein 39, c Myc
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 62-65 kDa, 50 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_2148585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


实验方案

Product Specific Protocols
WB protocol for c-MYC antibody 10828-1-APDownload protocol
IHC protocol for c-MYC antibody 10828-1-APDownload protocol
IF protocol for c-MYC antibody 10828-1-APDownload protocol
IP protocol for c-MYC antibody 10828-1-APDownload protocol
FC protocol for c-MYC antibody 10828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB

Nature

RIC-seq for global in situ profiling of RNA-RNA spatial interactions.

Authors - Zhaokui Cai
mouseWB

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
  • KD Validated
humanWB

Signal Transduct Target Ther

Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.

Authors - Xuewen Liu
humanWB,IF

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
  • KD Validated
WB

Cell Metab

Efferocytosis induces macrophage proliferation to help resolve tissue injury.

Authors - Brennan D Gerlach
  • KD Validated