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验证数据展示

  • FC experiment of HT-29 using CL555-21050

    FC experiment of HT-29 using CL555-21050

    1x10^6 HT-29 cells were surface stained with 0.4 ug CoraLite®555-conjugated EPCAM/CD326 Polyclonal antibody (CL555-21050) (red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

  • IF Staining of HT-29 using CL555-21050

    IF Staining of HT-29 using CL555-21050

    Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite®555 EPCAM/CD326 antibody (CL555-21050) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for IF/ICC, FC
Cat No. CL555-21050

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC

别名

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHT-29 cells
Positive FC detected inHT-29 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21050-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-21050 targets EPCAM/CD326 in IF/ICC, FC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human, mouse
免疫原 EPCAM/CD326 fusion protein Ag15393 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epithelial cell adhesion molecule
别名 EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1
计算分子量 314 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014785
基因名称 EPCAM
Gene ID (NCBI) 4072
RRIDAB_2923892
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16422
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
IF protocol for CL555 EPCAM/CD326 antibody CL555-21050Download protocol
FC protocol for CL555 EPCAM/CD326 antibody CL555-21050Download protocol
Standard Protocols
Click here to view our Standard Protocols