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验证数据展示

  • FC experiment of HT-29 using CL555-21050

    FC experiment of HT-29 using CL555-21050

    1x10^6 HT-29 cells were surface stained with 0.4 ug CoraLite®555-conjugated EPCAM/CD326 Polyclonal antibody (CL555-21050) (red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

  • IF Staining of HT-29 using CL555-21050

    IF Staining of HT-29 using CL555-21050

    Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite®555 EPCAM/CD326 antibody (CL555-21050) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for IF/ICC, FC
Cat No. CL555-21050

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC

Synonyms

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 ug/ml

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经过测试的应用

Positive IF/ICC detected inHT-29 cells
Positive FC detected inHT-29 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21050-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-21050 targets EPCAM/CD326 in IF/ICC, FC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity human, mouse
Immunogen EPCAM/CD326 fusion protein Ag15393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2923892
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16422
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
IF protocol for CL555 EPCAM/CD326 antibody CL555-21050Download protocol
FC protocol for CL555 EPCAM/CD326 antibody CL555-21050Download protocol
Standard Protocols
Click here to view our Standard Protocols