- Featured Product
- KD/KO Validated
CoraLite®555-conjugated EPCAM/CD326 Polyclonal antibody
EPCAM/CD326 Polyclonal Antibody for FC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HT-29 cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21050-1-AP
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-21050 targets EPCAM/CD326 in FC applications and shows reactivity with human, mouse samples.
Tested Applications | FC Application Description |
Tested Reactivity | human, mouse |
Immunogen | EPCAM/CD326 fusion protein Ag15393 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | epithelial cell adhesion molecule |
Synonyms | CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1 |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC014785 |
Gene Symbol | EPCAM |
Gene ID (NCBI) | 4072 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P16422 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL555 EPCAM/CD326 antibody CL555-21050 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |