验证数据展示

WB analysis of A549 using 20170-1-AP
WB result of KGA-Specific antibody (20170-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KGA-Specific transfected A549 cells.
IHC staining of human kidney using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).
Ready-to-use IHC kits available with KGA/GAC antibody included
IHC staining of human kidney using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).
Ready-to-use IHC kits available with KGA/GAC antibody included
WB analysis of HEK-293 using 20170-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody at dilution of 1:600 incubated at 4 degree celsius over night.
IF Staining of hela using 20170-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed hela cells using KGA-Specific antibody (20170-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
IF Staining of HEK-293 using 20170-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20170-1-AP (KGA-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis using 20170-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 20170-1-AP
IP result of anti-KGA-Specific (IP:20170-1-AP, 4ug; Detection:20170-1-AP 1:8000) with HEK-293 cells lysate 1440 ug.

Featured Product
KD/KO Validated

KGA-Specific Polyclonal antibody

KGA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20170-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Synonyms

GLS, KGA, GLS1, GAM, GAC

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (20)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, mouse kidney tissue, human heart tissue, human brain tissue, K-562 cells, mouse brain tissue, A549 cells, HeLa cells, rat brain tissue, HFF cells, MRC-5 cells
Positive IP detected in	HEK-293 cells, HFF cells
Positive IHC detected in	human kidney tissue, human brain tissue, mouse brain tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hela cells, HEK-293 cells

Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 18 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below

产品信息

20170-1-AP targets KGA-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	KGA-Specific fusion protein Ag14002 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	glutaminase
Synonyms	GLS, KGA, GLS1, GAM, GAC
Calculated Molecular Weight	669 aa, 73 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC038507
Gene Symbol	GLS
Gene ID (NCBI)	2744
RRID	AB_10665373
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94925
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

实验方案

Product Specific Protocols
WB protocol for KGA-Specific antibody 20170-1-AP	Download protocol
IHC protocol for KGA-Specific antibody 20170-1-AP	Download protocol
IF protocol for KGA-Specific antibody 20170-1-AP	Download protocol
IP protocol for KGA-Specific antibody 20170-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nat Med Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia. Authors - Daniel Herranz View Article
mouse	WB	Cell Stem Cell CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion. Authors - Gianluca L Russo View Article
human	WB	Blood Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. Authors - Nathalie Jacque View Article
	WB	Elife Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor Authors - Carolyn M Jablonowski View Article
rat	CoIP, WB	Int J Mol Sci Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase. Authors - Małgorzata Beręsewicz-Haller View Article
human	WB,IHC	EBioMedicine Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway. Authors - Binghua Li View Article

验证数据展示

WB analysis of A549 using 20170-1-AP

IHC staining of human kidney using 20170-1-AP

IHC staining of human kidney using 20170-1-AP

WB analysis of HEK-293 using 20170-1-AP

IF Staining of hela using 20170-1-AP

IF Staining of HEK-293 using 20170-1-AP

WB analysis using 20170-1-AP

IP experiment of HEK-293 using 20170-1-AP

KGA-Specific Polyclonal antibody

KGA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.